Journal: bioRxiv
Article Title: 3D osteocyte lacunar morphometry of human bone biopsies with high resolution microCT: from monoclonal gammopathy to newly diagnosed multiple myeloma
doi: 10.1101/2023.07.12.548656
Figure Lengend Snippet: (a-c) Representative light microscopy images of the bone marrow compartment from the biopsy samples: CD138 positive staining in brown, cell nuclei counterstain in blue. (d-f) 3D renders of low resolution (7 μm voxel) microCT scans of representative bone biopsies. (g-i) Quantitative analysis of trabecular bone strut thickness distribution and color code visualization with Dragonfly software. (j) Frequency distribution of strut thickness. Scale bars equal to 100 μm (a-c), 1 mm (d-f).
Article Snippet: The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
Techniques: Light Microscopy, Staining, Software